This study is aim to construct human liver tissue structure using 3D biological printing technology. We used the characteristics of stem cell differentiation and cell 3D printing technology to investigate the progress of stem cell differentiation, 3D printing process and training components of the environment. The biological properties of cell-cell and cell matrix in natural liver tissue were successful reproduction with realizing human liver cells mature and maintaining long-term function. In addition to this, bile duct network was widely distributed in the cellular structure, improving the three-dimensional structure of the material exchange ability, and ultimately the formation of long-term survival in vitro with drug metabolizing function of human liver tissue. The liver function of 3D printing human liver tissue in vivo was evaluated by using Fah-/- model mice with hereditary tyrosine degeneration. The results showed that the 3D printing liver tissue significantly prolonged the survival time and improve the liver function in mice. It can be concluded that the human liver tissue model based on 3D printing technology has the primary liver function of liver, which provides a new tool for drug research and disease mechanism research.