Detailed Abstract
[Liver Poster Presentation 4 - Transplantation]
[P035] The effects of various immunosuppressants to epithelial-mesenchymal transition on HCC cell line
Berik ROVGALIYEV1, Kwang Woong LEE2, Seung Cheol OH3, Kuyng Cheol YOON2, Suk Kuyng HONG2, Kyung-Suk SUH2
1General surgery, Seoul National University, Kazakhstan
2General surgery, Seoul National University, Korea
3General surgery, Seoul National University, Korea, Kazakhstan
Introduction : The purpose of this study was to elucidate the effect of various immunosuppressants to epithelial–mesenchymal transition (EMT), which strongly correlated with tumor growth and metastasis of HCC.
Methods : The effect of various immunosuppressive drugs will investigate on tumor growth and lymph node metastasis in a Balb/c nude mice model of HCC. Cell proliferation and invasion were monitored in vitro using the CCK8 and Matrigel Invasion Chambers, respectively. Levels of expression specific markers of EMT were measured using Western blots analysis.
Results : Various immunosuppresses showed differing results to the proliferation of HCC in vitro study. Treatment with high and low doses of Tacrolimus and Cyclosporine A in high doses resulted in increase in the invasive potential of SK-Hep1 cells by enhancing EMT in vitro and there was no significant effect of Sirolimus and MMF on cell migration and invasion. The Western blotting analysis revealed that Tacrolimus increased the levels of expression of TGF-β, N-Cadherin, ZEB1, Slug in the HCC tumor cells, while Cyclosporine A, Sirolimus and MMF did not have a significant effect on the expression of these proteins. The increase in expression level was especially pronounced in TGF-β, N-Cadherin, ZEB1 proteins when using high doses of Tacrolimus (100ng/ml) and moderately expressed, however, higher than other immunosuppressors using a low dose (5ng/ml) of the drug. The use of other immunosuppressors in different doses did not significantly affect the expression of all proteins.
Conclusions : Tacrolimus and high doses Cyclosporine A increased the invasive potential of HCC cells in our in vitro study.
Methods : The effect of various immunosuppressive drugs will investigate on tumor growth and lymph node metastasis in a Balb/c nude mice model of HCC. Cell proliferation and invasion were monitored in vitro using the CCK8 and Matrigel Invasion Chambers, respectively. Levels of expression specific markers of EMT were measured using Western blots analysis.
Results : Various immunosuppresses showed differing results to the proliferation of HCC in vitro study. Treatment with high and low doses of Tacrolimus and Cyclosporine A in high doses resulted in increase in the invasive potential of SK-Hep1 cells by enhancing EMT in vitro and there was no significant effect of Sirolimus and MMF on cell migration and invasion. The Western blotting analysis revealed that Tacrolimus increased the levels of expression of TGF-β, N-Cadherin, ZEB1, Slug in the HCC tumor cells, while Cyclosporine A, Sirolimus and MMF did not have a significant effect on the expression of these proteins. The increase in expression level was especially pronounced in TGF-β, N-Cadherin, ZEB1 proteins when using high doses of Tacrolimus (100ng/ml) and moderately expressed, however, higher than other immunosuppressors using a low dose (5ng/ml) of the drug. The use of other immunosuppressors in different doses did not significantly affect the expression of all proteins.
Conclusions : Tacrolimus and high doses Cyclosporine A increased the invasive potential of HCC cells in our in vitro study.
SESSION
Liver Poster Presentation 4
Poster / Exhibition Hall and Lobby(2F) 3/31/2018 3:10 PM - 3:50 PM