Introduction : Nonalcoholic fatty liver disease (NAFLD) is characterized by a wide spectrum of liver damages spanning from steatosis, nonalcoholic steatohepatitis (NASH), cryptogenic liver cirrhosis, even to hepatocellular carcinoma. Oxidative stress is a strong contributor to the progression from simple fatty liver to nonalcoholic steatohepatitis (NASH). Our aim was to investigate the effects of hydrogen water on liver NASH and the mechanism underling these effects.

Methods : A methionine choline deficient diet (MCD) was prepared for the mouse models. In our investigation we made two experimental groups as follows: (1) MCD diet + normal water group(n=12); (2) MCD diet + hydrogen water group(n=12). Both groups were fed for three different periods, which are 8 weeks, 12 weeks, and 16 weeks.

Results : The control group and hydrogen water group average concentration of ROS at 8 weeks (588?4 FORT units vs 164?; P=0.029), at 12 weeks (587?6 FORT units vs 163?; P=0.029) and at 16 weeks (439?76 FORT units vs 160?; P=0.029). From H&E staining the inflammation was vicissitudinous in control group at 8, 12, and 16 weeks (control group C:151.50 vs HW group HW:123.25; P=0.289)(control group C:104.33 vs HW group HW:84.75; P=0.077)(control group C:147.00 vs HW group HW:104.00; P=0.157).

Conclusions : Daily consumption of hydrogen water reduces nonalcoholic fatty liver disease and may be an effective treatment for NASH by reducing hepatic oxidative stress, apoptosis, and inflammation.