Detailed Abstract
[BP Oral Presentation 4 - Basic Research]
[BP OP 4-1] LncRNAH19 promotes EMT and stemness property in humam pancreatic cancer cells through STAT3 pathway
Feng WANG1,2, Xiaodong TIAN1, Yinmo YANG1
1Department of General Surgery, Peking University First Hospital, China
2Department of Endoscopy Center, Peking University First Hospital, China, China
Introduction : The long non-coding RNA H19 plays a crucial role in tumorigenesis of human pancreatic cancer (PC) cells. This study aimed to assess its role in the regulation of epithelial-mesenchymal transition (EMT) and the stemness property in PC cells.
Methods : Knockdown and overexpression were employed to manipulate H19 levels in PC cells, and serum-free floating culture systems were used to assess the sphere-forming ability. CCK-8 assay and xenograft assay were used to evaluate the chemosensitivity in vitro and in vivo. Migration and invasion ability was evaluated by transwell assays. The expression of stemness and EMT markers was detected by flow cytometry, qPCR and Western blot analysis. The status of STAT3 signaling was examined by qPCR and Western blot analysis.
Results : We found that down-regulating H19 expression in PANC-1 cells reduced tumor cells proliferation and chemoresistance both in vitro and in vivo. Meanwhile, the migration and invasion ability, the EMT process and the stemness property were significantly suppressed. In contrast, up-regulating H19 expression in CAPAN-1 cells yielded opposite results. Additionally, the modulation of H19 on EMT and stemness property was associated with the regulation of STAT3 activity.
Conclusions : We revealed that H19 regulates EMT and stemness property in human PC cells through regulating STAT3 pathway. These findings may offer potential therapeutic targets for PC patients with excessive H19 expression.
Methods : Knockdown and overexpression were employed to manipulate H19 levels in PC cells, and serum-free floating culture systems were used to assess the sphere-forming ability. CCK-8 assay and xenograft assay were used to evaluate the chemosensitivity in vitro and in vivo. Migration and invasion ability was evaluated by transwell assays. The expression of stemness and EMT markers was detected by flow cytometry, qPCR and Western blot analysis. The status of STAT3 signaling was examined by qPCR and Western blot analysis.
Results : We found that down-regulating H19 expression in PANC-1 cells reduced tumor cells proliferation and chemoresistance both in vitro and in vivo. Meanwhile, the migration and invasion ability, the EMT process and the stemness property were significantly suppressed. In contrast, up-regulating H19 expression in CAPAN-1 cells yielded opposite results. Additionally, the modulation of H19 on EMT and stemness property was associated with the regulation of STAT3 activity.
Conclusions : We revealed that H19 regulates EMT and stemness property in human PC cells through regulating STAT3 pathway. These findings may offer potential therapeutic targets for PC patients with excessive H19 expression.
SESSION
BP Oral Presentation 4
Room C 3/31/2018 9:20 AM - 10:10 AM